Cycle paths, trails and other cycling infrastructure in regional areas can apply for up to $15 million grants in Round Two of the Regional Development Australia Fund grants.
A new trail between Gawler and Tanunda in the Barossa region of South Australia was built under a grant in Round One.
Federal Regional Development and Local Government Minister Simon Crean announced Round Two earlier this month and said $200 million had been allocated for it.
Non-profit must have a minimum annual income of $1.5 million.
Projects in capital cities must show it will benefit the broader region or other parts of regional Australia.
Local Regional Development Australia committees will have a stronger role under changes to the program.
Details of the program are at: http://www.regional.gov.au/regional/programs/rdaf.aspx